Symptom
Possible cause Action
Buffer-pin valve does not move Polymer crystallizations have formed
around the buffer-pin valve
If you see any crystals, leaks, and
dried residue around the buffer-pin
valve, clean the valve and the array
locking lever immediately.
Add DI water to the buffer solution to
dissolve crystals.
Note: Use the lint-free swabs,
included in the PDP Cleaning kit
(Cat. No. 4414007 ).
If leaks persist, contact Thermo
Fisher Scientific.
Perform maintenance tasks routinely
as described in “Maintenance
schedule“ on page 223. If leaks
persist, contact Thermo Fisher
Scientific.
The vent hole behind the buffer-pin
valve is clogged
Clean the vent hole behind the buffer-
pin valve with DI water.
The PDP block is not in the correct
position
See "Polymer delivery pump (PDP) is
extremely noisy and vibrating while
running any wizard" . If the problem
persists, contact Life Technologies.
Polymer crystals on the buffer-pin
valve
Buffer valve leakage Clean the buffer-pin valve. Perform
maintenance tasks routinely as
described in “Maintenance
schedule“ on page 223.
Fluid does not move through the
polymer delivery pump and into the
ABC from polymer or conditioning
pouch
Blockage in fluid path or problem
with polymer delivery pump
Contact Thermo Fisher Scientific.
Poor signal and resolution after
replenishing polymer
The Check Valve is clogged (see
Figure 35.
Wash the channels using the Polymer
Delivery Pump Cleaning Kit (Cat.
No. 4414007 ). If the problem persists,
contact Thermo Fisher Scientific.
Any of the following visual or audible
conditions:
• Unstable current
• Arc-detect errors
• A crackling noise at the
beginning of electrophoresis
• A blue lightning symbol below
the oven
• An error message regarding
electrical current
The buffer level is below the fill line. Verify that buffer level is at or above
the fill line.
The buffer spilled on top of the CBC.
IMPORTANT! Ensur
e that the
environment (humidity) is non-
condensing.
Wipe away spills, moisture, and
c
ondensation with a lint-free lab
cloth. If the problem persists, contact
Thermo Fisher Scientific.
The buffer spilled on top of the
Aut
osampler.
Condensation on the CBC.
Condensation around the septa.
Appendix A Troubleshoot
Instrument troubleshooting
A
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
257
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