Symptom
Possible cause Action
“Leak error” occurs when capillary
arrays are filled with fresh polymer or
when replenishing polymer, causing
the wizard to fail to complete.
The Yoke is not seated properly on the
buffer-pin valve.
Make sure the buffer-pin valve lever
(yoke) is seated properly on the
buffer-pin valve (see Figure 34).
If the lever does not move up and
down freely, close the door. Restart
the instrument and the computer.
(see “Restart the instrument and the
computer“ on page 251).
After the instrument has restarted,
check the lever movement.
If the lever does not move up and
down freely, contact Thermo Fisher
Scientific.
If the lever moves up and down freely,
push the upper polymer block all the
way back against the wall.
• “Leak detected during polymer
delivery”
• “Leak detected during bubble
compression”
The run aborts.
Bubbles in the polymer system. Run the Remove Bubbles wizard to
clear bubbles.
Leak in the polymer system. Check for evidence of leaks.
If polymer leak occurred, conduct a
water wash and wash the pump trap
using the Polymer Delivery Pump
Cleaning Kit (Cat. No. 4414007 )
supplied with the instrument.
Buffer valve leakage. Check the buffer-pin valve and see if
it closes correctly.
Clean the buffer-pin valve.
Ensure that the maintenance
schedule is followed per 3500 Series
Data Collection Software 3.1
notifications.
Filling the array during install array. Run Fill the Array with fresh
Polymer wizard, or run Change
Polymer Type wizard.
“Bubble” error Bubbles present Run the Remove Bubbles wizard.
“Java update scheduler” error
message
The Java updater is unable to
complete the update.
Close the Java update scheduler.
Note: The Java update scheduler
does not affect the performance of
the 3500 Series Data Collection
Software 3.1 or the quality and
accuracy of the data collected.
Appendix A Troubleshoot
Error messages
A
260
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
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