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Applied Biosystems 3500 - Page 55

Applied Biosystems 3500
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Figure 6 8-s
trip fast tube assembly
1
Plate retainer
2
Septa
3
8-strip tubes
4
96-well tray
5
Plate base
1.
Place the tubes in the 96-w
ell tray.
IMPORTANT! The array tips will be damaged if the plate retainer and septa strip
holes do not align correctly.
2.
(8-strip standard tube only) Place the retainer on the tubes.
3.
Align the holes in the septa strip with the retainer (8-strip standard tube) or the
tubes (8-strip fast tube), then rmly press down.
4.
Place the tray-tube-retainer assembly into the plate base.
5.
Snap the plate retainer (cov
er) onto the plate, septa, and plate base.
6.
Verify that the holes of the plate retainer and the septa strip are aligned. If holes
are not aligned, re-assemble and then assemble the plate assembly.
7.
If the reagents of any well contain bubbles or are not located at the boom of the
well, briey centrifuge the plate, remove the plate from the centrifuge, and verify
that each sample is positioned correctly in the boom of its well.
Chapter 3 Set up and run
Prepare and load sample plates
3
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
55

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