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Applied Biosystems 3500 User Manual

Applied Biosystems 3500
326 pages
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diagram 51
display in softw
are 75
plot seings, change 97
plots, fragment
display plots 97
label peaks 98
overlay 98
scale 97
seings 97
zoom 97, 115
polymer
ll capillary array 234
handling 232
location 15
on-instrument limits 20
part numbers and handling 20
precautions for use 232
replenish 232
store 234
storing partially used pouch 234
Polynomial Degree 181
power requirements 286
pre-expiration warning messages 37
pre-heat oven 41, 108, 121, 131
preferences
advanced or basic setup 37
calendar reminder time 37
date format 37
le location 37
instrument name 37
library ltering 37
pause after last injection 37
plate type 37
reports 37
run logs 37
sequencing export 37
sequencing trace 37
sequencing trace print 37
suppress pre-expiration warning messages 37
system 37
table seings 36
warning dialogs 37
preview run
duplicate injection 62
injection list 62
start instrument run 64
primer peak, eliminating 181
print
audit reports 208
install check eport 130, 137
plate layout 50
sample quality reports 101
spatial calibration report 107, 119
spectral calibration 107, 120, 130, 137
trace quality reports 89
user report 200
probablility of error 88
processed plates 62
Pull-Up peak
ag 94
seings 184
pump
avoid damage 236
bubbles, remove 237
ush water trap 238
maintenance 236
parts of 252
remove bubbles 237
trap, ush 238
troubleshoot 254
wash chamber and channels 237
water trap, ush 238
PUP Score 84
pure base
color 37
QV range 37
pure base seings 88
purge
audit records 210
library 243
library entries 245
library items 243
Q
Q and Condition
113
QC protocol
create 185
dened
185
seings 187, 190
QC report, sequencing, generate 89
quality ags
fragment 67, 94
sequencing 67, 84
quality seings
fragment analysis 179
sequencing analysis 173
quality value
See also QV
probability of error 88
spectral calibration 113
See also QV
quarterly instrument maintenance 226
Quick Start 58
QV, probability of error 88
QV (average quality value), monitor run 67
See also average quality value [QV (average qual-
ity value)
Index
320
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1

Table of Contents

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Applied Biosystems 3500 Specifications

General IconGeneral
Number of capillaries8
Capillary Length50 cm
LaserSolid-state laser
Instrument TypeCapillary electrophoresis system
TechnologyCapillary Electrophoresis (CE)
Weight95 kg
Power Requirements100–240 V

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