Applied Biosystems 3500 User Manual

QV seings, sequence
basecalling protocol 173
color and ranges
37
monitor run screen 173
recommended ranges 88
QV20+
report 89
result 84
seing 178
R
re-inject samples
from Monitor Run screen 69
from Results screen 89, 101
re-injection
See also duplicate injection
allelic ladder 71
collect data for specic wells 69
dened 69
displayed in plate view 70
le location 155
samples, all in injection 69
samples, individual 69
troubleshooting 264
See also duplicate injection
re-injection buon dimmed 69
reactivate suspended account 220
Reactivate the Instrument maintenance wizard 242
Read Length 127
recent
plates 62
runs 62
red indicator light 17
related documentation 310
Remove Bubbles maintenance wizard 237
rename samples in review results 99
Replace with index term 103, 130
Replenish Polymer maintenance wizard 232
replicate injection. See injection list
report
e-sig 216
planned maintenance 227
report seings, sequence 89
reports
action log 207
audit 208
CRL and CRL distribution 89
default seings 37
e-sig 216
export sequencing 90
fragment results 101
install check 130, 137
plate grid 50
sample quality 101
sequencing results 89
spatial calibration 107, 119
spectral calibration 107, 119
system conguration history 206
trace score 89
user 200
requirements, environmental 287
reset buon on front panel 280
restore
audit records 210
library entries 244
library items 243
results, review for previously run samples 84, 94
See also review results, sequencing
results group
create new 153
dened 152
example 157, 158, 160
for HID applications 160
seings 155
results groups
assign to plate 77
create 153
folder 155
for HID applications 52, 152
store samples by plate name 157
results.. See review results
resume run 72
review results
currently running plate, fragment analysis 93
currently running plate, sequencing 83
import sample les 84, 94
previously run plate 84, 94
rename 99
show/hide columns 84
sort 84
review results, fragment
access 92
currently running plate, fragment 83, 93
label peaks 98
overlay plots 98
plots 97
quality ags 94
sample quality 94
scale 97
sizing curve, overlay 101
thumbnails 99
troubleshoot 267
zoom 97, 115
review results, fragment/HID
modify data in secondary analysis software 90,
102
normalized data 96
Index
3500/3500xL Genetic Analyzer User Guide—Data Collection Software v3.1
321

Applied Biosystems 3500 Specifications

General

Number of capillaries	8
Capillary Length	50 cm
Laser	Solid-state laser
Instrument Type	Capillary electrophoresis system
Technology	Capillary Electrophoresis (CE)
Weight	95 kg
Power Requirements	100–240 V