6 Analysis Flow Chart
6.1 Analysis Flow Chart
67
GC-2010 Plus
Q Glass insert (liner)
・ There are two types of glass inserts, one
for split analysis and the other for
splitless/WBI analysis. Select the correct
type based on the sample injection
method.
A glass insert for split analysis can be used
for splitless analysis. For the AOC-20i auto
injector, the silica wool is normally positioned
25 mm from the upper end of the glass
insert for split. On the other hand, the silica
wool is positioned at bottom of the glass
insert for splitless.
・ The quantity and position of the glass
wool in the insert directly affects the
reproducibility of results. For the AOC-
20i auto injector, the glass wool is nor-
mally positioned 25 mm from the upper
end of the glass insert.
・ Samples come into direct contact with
the inner surface of the glass insert and
the silica wool. On these hot surfaces,
unstable compounds may be decomposed
or adsorbed. If this occurs, use deacti-
vated lineas and glass wool.
Q Analytical column
・ Verify that carrier gas is flowing for enough time to flush the air in the column before
increasing the column oven temperature. Otherwise, the column liquid phase becomes
oxidized, and cannot separate compounds properly. This is especially important for polar
columns.
Press the [SYSTEM] key, and set a start time. This ensures that carrier gas flows for the
set time prior to temperature control of the heated zones.
・ Selection of the analysis column is very important in GC analysis. In general, select a
liquid phase whose polarity and chemical characteristics are similar to those of the
analysis target compound to obtain good peak shape. However, highly polar columns
require low temperatures and do not last long.
Therfore, when analyzing an unknown sample, begin by analyzing it on a neutral column
at higher temperatures. Switch to a more polar column if necessary.
Q Installing the analysis column
Install the column as described in sections 4.3, 4.4, and 4.5.
Use a box nut on the injection port side. Use a column nut on the detector side.
25mm
Silica wool
For split analysis For splitless/
WBI analysis
5.5mm
Fig. Correct placement of silica wool
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