Software Reference 190
V4.2 LabChip GX User Manual PerkinElmer
Analysis Tab (Continued)
Clear Default Ladder button
Displays at the bottom of the Ladder Sizes if a default ladder is
defined for the plate. (Not available for Protein Charge Variant
assays.)
Ladder Concentration
The concentration (in ng/uL) for the ladder peaks. (Not available for
Glycan or Protein Charge Variant assays.)
Standard Curve
Determines whether the fit for the standard curve used to calibrate
migration time to size will be done on a point-to-point, logarithmic,
or Polynomial fit of the ladder. DNA, gDNA, and Glycan assays are
set to point-to-point curve fits but can be changed to Polynomial.
RNA assays are set to point-to-point curve fits but can be changed
to Logarithmic. Protein assays are set to Log and cannot be
changed. (Not available for Protein Charge Variant assays.)
Data Range
• Baseline - Specifies the time in seconds after the start of the
run when the first peak can appear (any peaks appearing before
this time are ignored).
• Well End - Specifies the time when peak detection stops. The
graph ends at this time.
Dilution Ratio (Protein assays only)
Used to compensate for differences between sample and ladder
dilutions in the computation of the sample peak concentrations.
• Ladder - Reflects a dilution of ladder in a total volume of ladder
and sample buffer according to the LabChip GX/GXII Assay
User Guide.
• Sample - Reflects a dilution of sample in a total volume of
sample, water, and sample buffer according to the LabChip
GX/GXII Assay User Guide.
System Peak Exclusion (Protein and Glycan assays only)
Used to specify the region where System peaks are to be tagged
and excluded from the analysis.
• Ladder Ratio - Time at which ladder peaks are detected as
ladder peaks rather than system peaks. For example, if the
ladder ratio is set to 1.5, the software multiplies the lower
marker migration time x 1.5 and then begins ladder peak
identification. In this example, if the lower marker elutes at six
seconds, the ladder peak detection starts at 6x1.5 or 9 seconds.
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