Software Reference 176
V4.2 LabChip GX User Manual PerkinElmer
Peak Table View (Continued)
Area - The peak area is calculated as the sum of the parallelograms
of a point-to-point fit down to absolute zero minus the parallelogram
of the local baseline down to absolute zero. Data points are 0.05
seconds apart.
Barcode - The barcode on the plate used in the run.
Baseline - The center average height of the peak baseline,
measured in RFUs.
Chip ID - The ID number of the Chip used to run the plate.
Comment - Displays any comments associated with the well, either
from the Sample Name File selected in the Advanced Tab on the
Start Run Window or from the Sample Name Editor Window.
Conc. (ng/uL) - (DNA and Protein assays only) The concentration
calculated relative to the ladder and marker peak concentrations.
The ladder concentration is displayed in the Analysis Tab of the
Assay Analysis Window.
Corr. Area (Glycan assays) and Time Corr. Area (Protein assays) -
Displays the mobility corrected area. The area of a peak should
represent the total area of the compound responsible for the
fluorescence. In the LabChip instruments, the fluorescence of the
compound moving past the detector is sampled at constant time
intervals unlike the conventional slab gel where the compound is
spread out spatially and imaged at a single time. To convert from a
peak area integrated in the time dimension to one integrated in the
space dimension, we use the fact that x is proportional to µt so ∆x
α µ∆t and the mobility µ is proportional to 1/T where T is the
migration time of the peak. So the Time Corr Area = 100 * Area/T.
The 100 is an arbitrary constant designed to keep the corrected
area in a range similar to the original area computed from the time
series data.
Date_Time - The date and time that the plate data file was created.
Expected Fragment (BP or kb)/ Expected Protein (kDa)/
Expected Glycan (CGU) - Displays the size of the Expected
Fragments, Expected Proteins, or Expected Glycan.
Fragment Area - (RNA assays only) The sum of the trapezoids
between the fragment start and end times. The trapezoid base is a
line drawn across the fragment base from the start point to the end
point.
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