Software Reference 203
V4.2 LabChip GX User Manual PerkinElmer
Titer Tab (Continued)
• Name - Specifies a name to display in the Type description for
the peak.
Import Titer from Another Plate - Selecting the check box opens
the Select Plate For Titer Import window to select the desired titer
from another plate. The plate must be open in the collection, the
titer curve must be analyzed, and the normalization peak must
match. The normalization peak is set in the Analysis tab: Lower
Marker, Upper Marker (DNA only), or Sample Peak. If the Sample
Peak option is used, the peak size specified must also match. If no
compatible plate is found in the workspace, a message displays and
the Import is cancelled. Clearing the check box returns to the
manually entered titer.
Concentration to Favor in Fit - Adjusts the weight of the points in
the linear fit for the Titer Standard Curve. The points are weighted
by raising the concentration to a power depending on the slider
position. Each line on the slider represents a ½ power increment,
with the center position equal to 0. The left most position raises the
concentration to the power -2.5. The right most position raises the
concentration to the power of 2.5 to weight each point.
Force Fit through Origin check box - If selected, the Titer
Standard Curve is forced to include the origin (0,0). This prevents
very small peaks from generating negative concentrations.
Fit Model - Choose Linear or Quadratic to fit the Standard Curve to
the Titer. Linear: y = a + bx. Quadratic: y = a + bx + cx
2
.
Plot Titer Curve button - Plots the Titer Standard Curve using
linear regression to fit the data to a straight line and displays the
User Titer Window. Printing the Titer from the User Titer Window
prints a report that contains the Titer graph, the fit parameters (fit
model, concentration weight, origin option), fit coefficients, fit quality
(R^2), and the data table (normalized area vs concentration).
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