Data Analysis 103
V4.2 LabChip GX User Manual PerkinElmer
Raw Data
ASCII text file that contains the signal data from the run as one file
per well or multiple wells in the same file. Note that data that is
exported has been smoothed using the polynomial filter.
In addition to exporting time and value information, you can choose
to export Size information. This information is determined based on
aligned data and is used to correlate the peaks across different runs
or from one row to another.
When Include Size Data is not checked and only time and values
are exported, the state of the analysis function (peaks aligned or
not aligned with the ladder data) determines whether or not data
that is exported is or is not aligned. The same is true for the Zero
Baseline function: if enabled, data that is exported is also zeroed to
the baseline.
Below is an example of part of a raw data file exported from a DNA
assay (header not included and data truncated in this example):
DATA
Time, Size, Value
0.000,-158.365,0.80
0.017,-158.269,0.86
0.033,-158.173,0.53
0.050,-158.077,0.63
0.067,-157.981,0.45
0.083,-157.885,0.28
0.100,-157.788,0.24
0.117,-157.692,-0.05
0.133,-157.596,-0.38
0.150,-157.500,-0.66
0.167,-157.404,-1.06
0.183,-157.308,-1.45
0.200,-157.212,-1.70
…
Raw data can also be exported in a Chromatography Data
Interchange Format (formerly AIA format), which is used by some
graphical analysis software tools. The Include Size Data and Export
Single Table options are not available with Chromatography Data
Interchange Format.
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