Understanding Peak Finding 110
V4.2 LabChip GX User Manual PerkinElmer
The Baseline Plateau property defines the minimum length of
baseline region needed for computation of this average. Often, the
region between peaks may not be long enough to qualify as
baseline, in which case the baseline is drawn from the nearest
qualifying baseline region on each side of the peaks.
In Figure 48, the orange trace shows the signal slope superimposed
over the data. The light blue traces are the peak baseline. The
scale for the slope is the same as for the data but with units of /sec.
For these well defined peaks, a threshold of 15 /sec clearly extracts
the full peak signal.
Figure 48. Slope Threshold
The Slope Threshold is used to distinguish between baseline and
peak regions, so it should be set with care. A common mistake is to
set the threshold too low, making it difficult for the analysis to find
the baseline. On the same data signal, near the lower marker there
is sufficient noise to create variation in the slope values as shown in
Figure 49 on page 111.
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