Software Reference 206
V4.2 LabChip GX User Manual PerkinElmer
Advanced Tab (Continued)
Peak Integration Mode
Specifies the mode for determining the baseline.
• Peak Baseline - A global peak baseline is used as the baseline
for all peaks. This baseline is determined by stitching together
regions of low variance across the data signal.
• Valley to Valley - Each peak is assigned a baseline by drawing
a line under the peak joining the start and end of the peak at the
data signal values.
Smear Baseline
Specifies the method for determining the Smear Baseline. This
option only displays after a smear has been added in the Smear
Analysis tab. Not available for Protein Charge Variant assays.
• Two Point -The baseline is a single straight line between the flat
baseline areas at the beginning and end of the trace. This option
is preferred for very shallow smears where the signal slope
does not rise above the Peak Slope Threshold.
• Peak Baseline - The Smear baseline uses the local peak
baseline for the start and end points of the smear baseline. If the
signal slope is below the peak slope threshold, the local peak
baseline follows the signal.
High Conc Size Corr
Specifies the method of correction for the ladder migration time
when the sample concentration is significantly higher than the
ladder concentration. The ladder migration time is corrected
independently for each well to compensate for the increased
migration speed of high concentration samples. The desired option
can be selected in the Assay Editor window before the sample is
run, and cannot be changed after the sample is run.
• Disabled - The ladder migration time is not corrected
• Linear - The ladder migration time correction is proportional to
the sample to ladder concentration ratio.
• Nonlinear - The ladder migration time correction is a function of
the ratio of sample and ladder concentrations and saturates at
very high sample concentrations. (Default)
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