Software Reference 207
V4.2 LabChip GX User Manual PerkinElmer
Advanced Tab (Continued)
Smear Smoothing
Fits a smooth spline curve to the smear and replaces the actual
data signal with the spline curve. Smear smoothing smooths out
rough smears that can be generated by the DNA High Sensitivity
assay when evaluating very low concentration samples. If the
smear is very rough and a minor peak or noise spike is significantly
higher than the smear, the Size at Maximum property may be the
height of the spike, instead of the height of the smear. Use Smear
Smoothing and adjust the Threshold until the smear is smooth.
• Disabled - The actual data signal is used to calculate the smear
properties. (Default)
• Spline - A spline curve is fit to the data and replaces the actual
data signal for analysis.
— Threshold - Specifies how closely the spline curve
follows the data signal in the smear. The higher the
Threshold, the more closely the curve follows the data.
Marker Find
The Marker Drift Tolerance specifies a percent of migration time to
use when determining the correct marker peak. See (“Marker Drift
Tolerance” on page 313) for details. Not available for Protein
Charge Variant assays.
Smear Molarity Integration
The Integration Step Size specifies the time, in seconds at which to
divide a smear into segments. The size at the center of each
segment and the concentration of each segment is used to
calculate the smear molarity.
GQS Smear Start Size (gDNA Assays only)
Specifies the start size of the GQS Smear used to calculate Total
gDNA Concentration and Genomic Quality Score (GQS). Only
editable in the Assay Editor window when creating an assay.
After the sample is run, edit the start time in the Smear Analysis Tab
on the Assay Analysis window or by drag the start of the smear in
the Graph View.
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