Glossary of Terms 321
V4.2 LabChip GX User Manual PerkinElmer
RNA Degrade Factor
This value quantifies the degradation of the ribosomal peaks, which
typically leads to an increase in the fast area region between the 5S
and the 16S (prokaryote) or 18S (eukaryote) ribosomal peaks. The
quantity is computed as follows:
For prokaryote:
200 * Fast Area / (Fast Area Time Span) / (Height of 16S +
Height of 23S)
For eukaryote:
200 * Fast Area / (Fast Area Time Span) / (Height of 18S +
Height of 28S)
As the sample RNA degradation increases, this factor will increase.
A value below 10 is obtained with high quality RNA but the user
should determine their own threshold for discriminating between
good and bad RNA. This quantity can be viewed and reported in the
Well Table View.
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