Glossary of Terms 328
V4.2 LabChip GX User Manual PerkinElmer
Titer
Allows the use of a standard with a known concentration to be used
as a calibration standard instead of using a ladder. The well location
and known concentrations of the standard are specified in the
Assay Analysis Window on the Titer Tab.
The Titer Standard Curve used for calibration is normalized against
the lower marker (protein and RNA) or upper marker (DNA) to
adjust for slight variations in each well. A normalization standard
can be added to each well to be used only for normalization.
(Normalization standards must be the same concentration in each
well.) Specify the normalization standard in the Assay Analysis
Window on the Advanced Tab. Titers can also be imported from
another plate if the Normalization Standard is the same. If Sample
Peak is selected, the Sample Peaks must be the same size.
The Concentration to Favor in Fit adjustment on the Titer tab
enables you to choose whether to weight either the higher or lower
concentrations more heavily when calculating the Titer Standard
Curve.
Tool Tip
A small box containing text that describes the item indicated by the
mouse pointer. To view a Tool Tip, position the cursor over an item
on the window. Leave the cursor stationary for a moment and a
Tool Tip (if one exists for that item) displays.
Upper Marker
An internal standard that is added to a DNA sample in a well to
assist in determining size and concentration of the sample. The
marker is the same as the last peak in the DNA ladder.
Workspace
Use a workspace file to view the plate data from a run. Multiple
plate data files can be opened in the same workspace to enable
comparison between data from different plates or different runs.
Each workspace can contain multiple Collections for viewing the
same data in different layouts.
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