Data Analysis 46
V4.2 LabChip GX User Manual PerkinElmer
How the Software Analyzes Protein Data
(Continued)
4 A sizing ladder (see Figure 11), which is a mixture of protein
fragments of different known sizes, is run from the ladder vial
before and after every 12 samples. The concentration and sizes
of the individual proteins (in kDa) in the ladder can be changed
on the Analysis Tab.
Figure 11. Ladder Graph - Protein
5 The ladder is analyzed (see “Ladder Analysis” on page 311) and
a standard curve of migration time versus mobility is plotted
from the ladder by interpolation between the individual protein
size/migration points. The standard curve derived from the data
of the ladder well should resemble Figure 12.
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