PerkinElmer LabChip GX - Page 51

Data Analysis 51
V4.2 LabChip GX User Manual PerkinElmer
How the Software Analyzes RNA Data
(Continued)
7 A dye matching the lowest peak in the ladder is run with each of
the samples. This lower marker, labeled LM in the RNA sample
(see Figure 16) is used to align the ladder data with data from
the sample wells.
Figure 16. Markers - RNA
8 The standard curve and the markers are used to calculate RNA
fragment sizes for each well from the migration times measured.