Basic Operations of the Plate Washer
DSX™ System Service Manual 12-5
12.4.2 Purge
The Purge step is used to dispense wash fluid to the Dispense wash pins to remove any air
bubbles that may be in the pins. This step is usually performed at the start of a wash protocol
and when the washhead is positioned over the purge tray.
Table 12-1 The Purge Step
Step Operation
1 Move the wash head back to the X home position (over the purge tray)
2 Run the dispense pump to deliver the required volume at the “Purge
Pump Pressure” speed
3 Move the wash head to the pre-defined purge height
4 Wait 1 second
5 Energize the dispense valve for the required duration
6 Wait 1 second
7 Move the wash head back up to the home position height
12.4.3 Dispense
The Dispense step is used to deliver the indicated volume of wash fluid (range 50-999 µL)
into the wells and then aspirate the wash solution from the wells. If a bottom wash is desired,
the Wash Head is lowered to the bottom wash position so that the fluid will be aspirated from
the bottom of the wells while the fluid is being dispensed. The dispense height for a particular
plate is specified during system configuration. When the Dispense operation is called, the
steps indicated in Table 12-2 are performed:
Table 12-2 The Dispense Step
Step Operation
1 Check that the Waste Bottle is not full and that the vacuum pump is
functioning
2 Run the Dispense Pump to deliver the required volume of fluid
3 Perform 1 aspirate step
4 If Bottom Wash is required, move the Wash Head to the appropriate position
5 Move the Wash Head back up to the Dispense height
6 Energize the Dispense valve for the required duration
7 Move the Wash Head back up to the home position height
Dispense and Aspiration steps can be combined, but an aspiration step is not always a part of
a dispense step.
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