Dynex DSX - Page 92

The Z-Drive and the Pipette Module
6-14 DSX™ System Service Manual
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6.3.2.1. Functional
The following scenario describes the main function of the Z Pipette module.
When the XY arms have repositioned the Pipette module over a tip container, the Z
component moves the Eject component up and pipette down so that the spigot is
pushed into a new tip, thus loading a new tip. The Z component is then moved up,
with the loaded tip.
When the XY arms have repositioned the Z Pipette module over a fluid container,
such as a bottle or sample tube or deep well, the Z component moves the tip down into
the fluid. The fluid level is detected by using the pressure sensor to detect the change
in pressure when the tip enters the fluid.
The Pipette component then aspirates the required amount of fluid into the tip. The Z
component may move down to track the falling level of the fluid, if required, while the
fluid is aspirated. The Z component is then moved up, with the tip loaded with fluid.
When the XY arms have repositioned the Z Pipette module over the required fluid
container, such as a microplate well or deep well, the Pipette component dispenses the
required amount of fluid into the well. The XY arms then dispense some fluid to the
next well, and so on.
When all operations with the current tip have been performed, the XY arms reposition
the Z Pipette module over the used-tip container, and the Eject component pushes the
tip down so that it falls off the spigot, thus ejecting the tip.
When the XY arms have repositioned the Z Pipette module in front of the required
plate carrier, the Z component moves the module down. Then the Y motor moves the
Z Pipette module inwards, to engage in the slot of the plate carrier. Then the Eject
component is moved down so that it clamps the plate carrier on the spigot. The Z
component is then moved up, holding the plate.
When the XY arms have repositioned the Z Pipette module at the required destination,
the Z component moves down to position the plate in the carrier holder. The Eject
component moves up so as to disengage the plate carrier, thus loading the plate carrier
into the new position. The XY arms then move the Z Pipette module away without the
plate.