Replacing External Components
DSX™ System Service Manual 13-9
To Calibrate a Dispense Pump:
Note: The following materials are required to calibrate a pump:
- Calibrated gravimetric balance with 0.01g resolution (minimum
range:0.01 - 100.00g)
- Deionized water or suitable wash buffer
- Clean, flat bottom microplates for use in the Verification test
- A plate-sized, low-sided vessel capable of containing approximately
50ml of fluid without spillage (microplate wells are too small to
contain the volume of fluid used in the pump calibration).
1 Load the Pump Calibration software.
2 Select the Pump letter.
3 Purge the washer until it is verified visually that no air bubbles are present in the
fluid line between the dispense bottle and wash head.
4 Follow the instructions provided by the program. When Perform First Dispense? is
displayed, answer Yes.
5 Follow the instructions provided by the program. When Perform Second
Dispense? is displayed, answer Yes.
6 The program will indicate if the slope and intercept values are acceptable.
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The slope should be between 0 and 6.5 (typically this value will be in the
range of 1.5 to 2.5).
The intercept should be between 0 and 512 (typically this value will be in the
range of 25 to 40).
7 If the calibration is acceptable, select Yes to write changes to EEPROM.
Note: After new slope/intercept values for any pump are saved to the
EEPROM, the DSX must be powered off and back on to use those values
before any additional testing is performed on that pump.
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