SAMPLE PROCESSING, Capillary Mode Procedures
Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-25
(2) Press [ENTER]. The next sample No. is set and the Sample No. Setting screen
will disappear. The input sample No. will be displayed in the Next No. column in
the top line of the LCD.
CAUTION: Do not use zero as the sample ID number. If the ID number is set to
zero, the analysis results will neither be stored nor output. If the
sample I
D number is set to zero, continuous short beeps will sound
during sample aspiration to alert the operator.
NOTE: • The sample ID can include numbers (0-9) and hyphens up to a
maximum of 13 characters.
• Hyphens cannot be placed at the beginning or end of the sample ID
number, nor can two hyphens be placed consecutively.
• A number will not be carried beyond a hyphen. For example, the
number following 2-999 will be 2-000; 1-99-000 will follow 1-99-999.
• The number following 9999999999999 will be 1.
4 . Prepare 1:5 Dilution of Sample
Prepare a 1:5 dilution using the following materials:
• Diluent (CELLPACK)
• Microtube (MT-40, etc.)
• 40 µL pipette (40 µL SMI micropettor, mode 1058-D40)
• 160 µL pipette (50 µL-250 µL SMI micropettor, model 1200-J, etc.)
• Diluent dispensing container (beaker, etc.)
• Diluent dispensing equipment (pipettor, etc.)
(1) Rinse a beaker with CELLPACK, to eliminate dust, etc.
(2) Carefully dispense CELLPACK into the beaker with a pipettor.
(3) Carefully transfer 160 µL of CELLPACK from the beaker with a micro pipettor to
the capillary analysis container (e.g. Micro Centrifuge Tube).
(4) Pipette 40 µL of patient blood to the capillary analysis container containing the 160
µL of CELLPACK.
(5) Cap and mix well.
NOTE: Prepare and analyze the 1:5 sample dilution as soon after collection as
possible to minimize platelet agglutination. Also, if diluent is dispensed
ahead of time, measurement errors are generated because of
evaporation and contaminants; therefore, prepare new diluted sample
each time an analysis is performed.
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