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IPN 074-397-P1G
HAPSITE Smart Operating Manual
Chapter 12
Target Compound Methods
12.1 Introduction To Quantitative Analysis
Quantitative analysis is the process of building a method calibration library of target
compounds, analyzing samples and converting target compound responses to
concentration results. The process of building a quantitative analysis method
includes the following steps:
1 Preparation of a standard or standards at multiple concentrations.
2 Collecting representative data files of the compounds at one or more
concentration levels.
3 Identifying the compounds and building a target compound library.
4 Calibrating the library.
5 Collecting and processing unknown samples for target compounds.
The Calibrate function of the HAPSITE software is used to set up or define the
quantitative method. The ID Unknowns function is used to process and review
results from a quantitative method. Quantitative results are automatically displayed
on the HAPSITE front panel display after data collection.
The process of quantitative analysis by developing a HAPSITE method to collect
the standard data. This method must meet the chromatography performance
requirements of the application, e.g. retention times, compound separations,
sensitivity, etc., and will be used throughout the process of building the library and
processing results. Refer to Chapter 11, Method Editor for additional information
on building a method.
The library is the key component in the quantitative method. The library contains
three types of information; the retention time (time for the compound to elute from
the column), mass spectral data used to confirm compound identity, and the
response factor that is used to calculate the concentration of the compound.
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