13 - 9
IPN 074-397-P1G
HAPSITE Smart Operating Manual
6c Inject the desired amount of Internal Standard/Surrogate mixture into the
sample through the small end of the sample syringe. See Figure 13-6.
Figure 13-6 Injecting the Mix into the Sample Syringe
For calibration standard preparation, injection of the proper amount of a calibration
mixture is performed to achieve the desired compound concentrations. Rinse a
separate 10
L syringe with methanol three times (discard the methanol from each
rinse), rinse once with the calibration mixture (discard this as well). Inject the
desired amount of the calibration mixture through the small end of the sample
syringe to complete the calibration standard.
The following is a set of examples for amounts of standard mix (at 200 and 2000
g/mL) to be injected into 20 mL of water to achieve the stated concentrations. See
Table 13-1, Example Calibration Concentration Table, on page 13-9.
Table 13-1 Example Calibration Concentration Table
Concentration Desired Amount (at 200 µg/mL) to be injected
20 PPB 2
L
50 PPB 5
L
100 PPB 10
L
If using a 2000 µg/mL standard mix:
400 PPB 4
L
1000 PPB 10
L
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