Thermo Scientific appliedbiosystems SeqStudio Flex Series - Understanding Quality Values (Qvs)

Table 4 Well details (continued)
Result Description
Spectral calibration status For information, see “Auto-spectral calibration during an injection” on page 150.
Export status Indicates whether the results were saved to a location in addition to the instrument,
either during data collection or by exporting the Run history.
Understanding Quality Values (QVs)
Quality value ranges
The color of a QV bar indicates the QV of a basecall. The height of the QV bar indicates the relative
value of a QV. A taller bar equates with a higher, better QV.
•
Pure basecall with QV ≥20
•
Pure basecall with QV 15–19
•
Pure basecall with QV <15
•
Mixed basecall
Pure basecall versus mixed basecall QVs
•
Pure basecalls and mixed basecalls have the same probability of error for the associated basecall
(Quality Value (QV) = –10Log
10
(Pe), where Pe is Probability of Error.
•
High-quality pure basecalls typically have QVs of 20 or higher.
•
The distribution of quality values for mixed basecalls diers dramatically from that of pure
basecalls.
•
Mixed basecalls have a maximum QV of 20.
•
Review all mixed basecalls.
Quality values (QV) and probability of error (Pe)
QV
Pe QV Pe
1 79.0% 30 0.10%
5 32.0% 35 0.032%
10 10.0% 40 0.010%
15 3.2% 45 0.0032%
20 1.0% 50 0.0010%
25 0.32% 60 0.00010%
Chapter 7 View results on the instrument
Sequence analysis results
7
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SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide