Thermo Scientific appliedbiosystems SeqStudio Flex Series - QC Error and Alert Troubleshooting; QC Errors-Sequencing and Fragment Analysis

QC error and alert troubleshooting
QC errors—Sequencing and fragment analysis
See also “Sample QC and quality alerts” on page 172.
QC error Description Action
Analysis failure due to signal
strength is too low for analysis.
Sample is not present in the well. Ensure sample is present in the well.
Sample is not properly prepared. Ensure that samples are properly
prepared.
Injection run time or voltage is too
low.
Edit the run module to increase run
time or voltage. See “Run modules
library” on page 272.
Oscale peaks. Oscale peaks:
Adjust the injection parameters
and/or the sample concentration.
The sample has a small number of
peaks that are oscale.
If you are not using a factory-provided
dye set, turn on the O-scale
Recovery function in the dye set.
(This function is enabled by default
in factory-provided dye sets.) For
information, see “Dye sets library” on
page 285.
The sample concentration is too
high.
Dilute the sample, prepare a new
plate, then start a new run.
Quantitate the sample before adding
reagents for capillary electrophoresis.
Re-inject the samples with adjusted
injection conditions.
Injection time, voltage, or a
combination of these conditions can
be adjusted.
Spectral calibration dye matrix
is significantly dierent from
expected. Tap the help icon for
complete information on this quality
flag.
The incorrect dye set definition is
specified in the plate file.
Select the dye set definition that
matches the dye set chemistry used
for the samples.
Appendix A Troubleshooting
QC error and alert troubleshooting
A
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
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