Thermo Scientific appliedbiosystems SeqStudio Flex Series - Fill Array with Polymer Maintenance Wizard; Remove Bubbles Maintenance Wizard

Change Polymer Type—Flush array and spatial calibration
The final step in the wizard is to flush the capillary array with polymer and perform a spatial calibration.
A spatial calibration associates the signal from each capillary to a specific position on the CCD camera.
Tap Flush array and spatial calibration.
Wait ~15 minutes for the flush array and spatial calibration procedures to complete.
Fill Array with Polymer maintenance wizard
Fill Array with Polymer
Use the Fill Array with Polymer maintenance wizard to fill the capillary array with polymer. This wizard
can be useful for troubleshooting.
Note: The capillary array is automatically filled with polymer during each injection and when running
other maintenance wizards that aect the pump.
Item Requirements
Time required ~5 minutes
Materials required None
Tap Next to continue.
Remove Bubbles maintenance wizard
Remove Bubbles—Time and materials required
Use the Remove Bubbles maintenance wizard to remove bubbles from the fluid pathway. This function
is automatically performed during other wizards as needed.
Run this wizard if you observe bubbles in the fluidic path.
IMPORTANT! Running this wizar
d consumes polymer. Do not run this wizard excessively.
Note: A polymer pouch includes a small r
eserve volume that is used for the Remove Bubbles
maintenance wizard, which consumes ~350 μL of polymer. The reserve volume is sucient to run the
wizard ~4 times (including the remove bubbles step during other maintenance wizards). If you manually
run the Remove Bubbles maintenance wizard >4 times, the volume of polymer that is available for
samples may be depleted.
Item Requirements
Time required ~7 minutes
Materials required None
Tap Ne
xt to continue.
Chapter 13 Maintain the Instrument
Maint
enance wizard procedures
13
SeqStudio
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Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
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