Thermo Scientific appliedbiosystems SeqStudio Flex Series - Reactivate Instrument Maintenance Wizard

Reactivate Instrument maintenance wizard
Reactivate Instrument—Time and materials required
Use the Reactivate Instrument maintenance wizard to prepare the instrument for operation after
storage.
This wizard assumes that the Shut down instrument wizard was run before storage, that the pump and
channels are filled with conditioning reagent, and that no capillary array is installed.
Item Requirements
Time required ~50 minutes
Materials required
•
Capillary array
•
Anode Buer Container (ABC)
•
Cathode Buer Container (CBC)
•
Polymer, ABC, and CBC equilibrated to room temperature (15–30°C)
•
Conditioning reagent pouch
•
Purified water (distilled or deionized)
•
Lint-free lab wipes
•
Lint-free swab
•
20 mL Leur lock syringe
Tap Next to continue.
Reactivate Instrument—Fill the water trap and install the conditioning reagent
pouch
In this procedure, you flush the water trap, replace the installed conditioning reagent pouch with a fresh
conditioning reagent pouch, empty the waste ABC, and remove the array port plug.
1.
Allow the refrigerated polymer, ABC and CBC to equilibrate to ambient temperature (15–30°C)
before use.
2.
Open the instrument doors.
3.
Flush the water trap. For information, see “Flush the water trap (pump trap)” on page 379.
4.
Remove the installed conditioning reagent pouch by pressing the polymer pouch lever down.
5.
Wipe the pouch connector on the instrument with a moistened lab wipe.
6.
Install the new conditioning reagent pouch:
a.
Remove the seal from the conditioning reagent pouch.
b.
With the label facing the rear of the instrument, insert the pouch fitting into the slot on the
pump lever mechanism.
c.
Push the polymer pouch lever up.
Chapter 13 Maintain the Instrument
Maint
enance wizard procedures
13
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