System dye sets versus custom dye sets
System dye sets are available from Thermo Fisher Scientific. See “Fragment analysis reagents” on
page 541.
System dye sets
Fragment analysis dye sets Sequence analysis dye sets
•
D (DS-30, DS-31)
•
E5 (DS-02)
•
F (DS-32)
•
G5 (DS-33)
•
J6 (DS-36)
•
J6-T (DS-37)
•
E_BigDye
™
Terminator v1.1
•
Z_BigDye
™
Terminator v3.1
•
Z_BigDye
™
Direct
Custom dye sets are any dyes that are not available from Thermo Fisher Scientific or any user-created
dye set definitions. For example, if you open a factory-provided dye set and make a copy of it, the copy
is considered a custom dye set.
Note: If an application uses a subset of the dyes in a factory-provided dye set, you can still use the
factory-provided dye set definition. For example, if your application uses only two of the dyes in the
DS-33 Matrix Standard Kit (Dye Set G5), you can perform the spectral calibration using the DS-33
Matrix Standard Kit (Dye Set G5) and the factory-provided G5(DS-33) dye set definition.
Using BigDye
™
Direct Cycle Sequencing Kit chemistry
The dyes that are used in the BigDye
™
Direct Cycle Sequencing Kit are spectrally equivalent to the
dyes used in the BigDye
™
Terminator v3.1 Cycle Sequencing Kit. Therefore, when running a spectral
calibration (or performing an install run and saving the spectral calibration), you use BigDye
™
v3.1
chemistry.
The BigDye
™
Direct dye set is automatically calibrated when you generate a spectral calibration using
either of the methods stated previously.
When you perform a spectral calibration, install run, or create a plate file for use with the BigDye
™
Direct
Cycle Sequencing Kit, select the appropriate chemistry or dye set for each type of run:
•
Spectral calibration with matrix standards—Z_BigDye Terminator v3.1 dye set
•
Install run from which you save the spectral calibration—BDT v3.1 chemistry
•
Regular run with samples—z_BigDye Direct dye set
Chapter 12 Run calibrations and install checks
Run a spectral calibration
12
312
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
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