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Thermo Scientific appliedbiosystems SeqStudio Flex Series - Inspect the Data-Troubleshooting Procedures; View the Raw Data and the EPT Plot; Check Size Standard Quality (Fragment Analysis)

Thermo Scientific appliedbiosystems SeqStudio Flex Series
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Tap REMOTE SESSION to return to the remote support session.
Tap View to return to the chat.
6.
To end the remote support session, tap the active session, then tap Disconnect.
A Technical Support representative can also end the remote support session. REMOTE SESSION
is not displayed after a session has been disconnected.
Inspect the data—Troubleshooting procedures
View the raw data and the EPT plot
View the raw data and EPT plot to troubleshoot issues with a run.
The EPT view (Electr
oPhoresis Telemetry) shows instrument data conditions (current, temperatures,
electrophoresis voltage) as a function of time.
This EPT plot is useful for troubleshooting problems with specific runs.
1.
In the home screen, tap
Actions4Run hist
ory.
2.
Tap the plate of interest, then tap View.
3.
Tap a well, tap View, then tap the Raw tab.
4.
Tap the EPT tab.
Check size standard quality (fragment analysis)
This procedure describes how to check the size standard quality in the instrument software.
1.
In the home scr
een, tap the plate position for an active run, or tap
Actions4Run hist
ory.
2.
Tap a fragment analysis plate name, then tap View.
QC and SQ values are listed for each well. For information on how these values are determined,
see “Sample QC and quality alerts” on page 172.
A
QC indicates all QC criteria were met. An SQ of >0.75 is a passing result.
If any wells have a
or QC (indicating that a quality alert was triggered), or an SQ of ≤0.74,
r
eview the data and the details for the well as described below.
Appendix A T
roubleshooting
Inspect the data—Troubleshooting procedures
A
476
SeqStudio
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide

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