Thermo Scientific appliedbiosystems SeqStudio Flex Series - Instrument Troubleshooting

Instrument troubleshooting
Observation Possible cause Recommended action
The instrument lost power
A power failure occurred. Restart the instrument. See “Power o or
power on the instrument” on page 485.
A run that was in progress at the time of the
power failure must be restarted.
Run a control sample or perform an install
run to ensure that the consumables have not
degraded.
Replace the consumables if the results with
a control sample show that the consumables
have degraded.
The electrophoresis failed
or Current check failed is
displayed
Bubbles are present in the
pump.
Run the Remove Bubbles wizard. See
“Remove bubbles from the polymer pump
(wizard)” on page 377.
The Cathode Buer Container
has been installed on the
instrument for >2 weeks or
used for more than the allowed
number of injections (see
“Buer storage, usage, and
limits” on page 26).
Replace the Cathode Buer Container.
There is insucient cathode or
anode buer.
Check the fill line on the ABC and the CBC to
ensure that the buer is above the fill line. See
“Check buer fill levels” on page 70. Replace
a buer if the buer is at or below the fill line.
The septum on either the
Cathode Buer Container or
the sample plate is not
installed correctly.
Ensure that the septa are fully inserted into
both the Cathode Buer Container and the
sample plate.
Buer or other liquid was
spilled on top of the reservoir
septum or on top of the
autosampler.
Wipe the spill with a lint-free cloth.
There is condensation on the
Cathode Buer Container or
around the reservoir septum.
Wipe the condensation with a lint-free cloth.
Ensure that the humidity in the lab is non-
condensing.
The electrophoresis current
is fluctuating or unstable,
or Current check failed is
displayed
A bubble is present in the
polymer path.
Run the Remove Bubbles wizard. See
“Remove bubbles from the polymer pump
(wizard)” on page 377.
Arcing has occurred in the
pump. Look for black scorch
marks on the pump block.
Contact Technical Support.
Appendix A Troubleshooting
Instrument troubleshooting
A
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
499