Thermo Scientific appliedbiosystems SeqStudio Flex Series - Size Standards Library; Size Standard Overview; Size Standard Definition Files Provided

Size standards library
Size standard overview
A size standard defines the sizes of known fragments. It is used to generate a standard curve. The
standard curve is used to determine the sizing of unknown samples.
For example, the GS600 LIZ size standard in the software is used for GeneScan
™
600 LIZ
™
Size
Standard. It is used to generate a standard curve. The standard curve is used to determine the sizing of
unknown samples.
Size standard definition files provided
The library contains factory-provided size standard definition files.
For information on selecting a size standard for your application, see the DNA Fragment Analysis by
Capillary Electrophoresis User Guide (Pub. No. 4474504).
Note: The GeneScan
™
600 LIZ
™
Size Standard and the GeneScan
™
600 LIZ
™
Size Standard v2.0
contain the same peaks. The GeneScan
™
600 LIZ
™
Size Standard v2.0 can be used for size standard
normalization. For more information, see “Size standard normalization feature” on page 278.
Size standard definition For use with GeneScan
™
size standard Analysis range
GS1200LIZ GeneScan
™
1200 LIZ
™
Size Standard 20–1,200 bp
GS120LIZ GeneScan
™
120 LIZ
™
Size Standard 15–120 bp
GS500(-250)LIZ
[1]
GeneScan
™
500 LIZ
™
Size Standard 35–500 bp
GS500(-250)ROX
[1]
GeneScan
™
500 ROX
™
Size Standard 35–500 bp; the 250 bp peak is
not included
GS600_LIZ_(60-460) GeneScan
™
600 LIZ
™
Size Standard 60–460 bp
GS600_LIZ_(60-460)+Normalization GeneScan
™
600 LIZ
™
Size Standard v2.0 60–460 bp with size standard
normalization
GS600_LIZ_(80-400) GeneScan
™
600 LIZ
™
Size Standard 80–400 bp
GS600_LIZ_(80-400)+Normalization GeneScan
™
600 LIZ
™
Size Standard v2.0 80–400 bp with size standard
normalization
GS600LIZ GeneScan
™
600 LIZ
™
Size Standard 20–600 bp
GS600LIZ(60-600) GeneScan
™
600 LIZ
™
Size Standard 60–600 bp, for applications that
have primer peaks that obscure
the 20-mer and 40-mer peaks of
the GS600 size standard
Chapter 11 Manage library resources on the instrument
Size standards library
11
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
275