Prepare the samples
■
Sample preparation guidelines ......................................................... 73
■
Plate and strip-tube layout ............................................................. 75
■
Prepare and assemble sample plates ................................................... 76
Sample preparation guidelines
Item Guidelines
Plates and tubes
•
Use MicroAmp
™
Optical Reaction Plates or MicroAmp
™
Reaction Tubes with a
base and retainer set. Use a base and retainer with the same catalog number.
•
Use the appropriate septa for plates and tubes.
•
See “Plates bases retainers and septa” on page 539 for more information.
Fragment analysis sample
pr
eparation
•
P
repare the fragment analysis reactions as recommended by the kit instructions.
•
Use a 10–20 µL sample volume for 96-well plates or 8-strip tubes. Use a 5–20 µL
sample volume for 384-well plates.
•
Samples prepared with Hi‑Di
™
Formamide are stable for 16–24 hours on the
instrument. Samples that are prepared in aqueous solution have lower stability
than samples that are prepared in Hi‑Di
™
Formamide.
Note: Data quality may be reduced if samples exceed 16–24 hours on the
instrument. The software displays an alert if a sample will exceed 24 hours on
the instrument by the end of the run.
•
Ensure that Hi‑Di
™
Formamide is fresh.
–
Hi‑Di
™
Formamide should not undergo more than 8 freeze-thaw cycles. See
“Hi‑Di
™
Formamide storage and usage” on page 28.
–
Use the same day after thawing.
•
For more information, see DNA Fragment Analysis by Capillary Electrophoresis
User Guide (Pub. No. 4474504).
3
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
73
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