Thermo Scientific appliedbiosystems SeqStudio Flex Series - Pre-Run Check Messages and Plate Alerts During a Run; Pre-Run Checks When a Plate Run Starts or a Re-Injection or a Cancelled Injection Is Added to the Run Queue; Pre-Run Checks before each Injection or When a Paused Run Is Resumed

Pre-run check messages and plate alerts during a run
Pre-run checks are performed at the following times during a run:
•
When a plate run starts
•
When a re-injection is added to the Run queue
•
Before each injection
•
When a paused run is resumed
•
When a cancelled injection is added back to the Run queue
A partial list of pre-run checks is shown below.
•
Checks for valid spatial and spectral calibrations.
•
Checks for a passing install run.
•
Checks consumables expiration, volume, and number of remaining injections.
•
Determines if adequate storage space is available for results.
•
Determines if the plate has been installed in the drawer for more than 24 hours.
•
Checks that the save locations specified in the plate file are available and writable.
•
If you run using the automated barcode workflow, performs additional checks. See “Run plates
with the automated barcode workflow” on page 147.
Pre-run checks when a plate run starts or a re-injection or a cancelled
injection is added to the Run queue
Pre-run checks are performed when a plate run starts or when a re-injection or a cancelled injection
is added to the Run queue. If these pre-run checks do not pass, a message screen is displayed. Two
message types are possible:
•
Pre-run check failure message—The error condition must be corrected before the run can start.
•
Pre-run check warning message—For information only, tap OK to start the run.
Examples of pre-run check failure messages include "No spatial calibration available for capillary
array", "Plate type of the installed plate does not match the plate file".
Examples of pre-run check warning messages include "Injections on plate will exceed or have
exceeded 24 hours on instrument", "Network drive save location is not writable".
Pre-run checks before each injection or when a paused run is resumed
Pre-run checks are also performed before each injection or when a paused run is resumed. If these
pre-run checks do not pass, a
!
(Plate alert) symbol is displayed at the top of a plate position.
To display the reason for the alert, tap the
!
(Plate alert) symbol. The Notifications screen is
displayed. For more information, see the following sections:
•
“View alert and notification details” on page 149
•
“Plate alert troubleshooting” on page 507
Chapter 6 Start and monitor a run
Pre-run check messages and plate alerts during a run
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SeqStudio
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Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide