Thermo Scientific appliedbiosystems SeqStudio Flex Series - Spatial Calibration Troubleshooting

Observation Possible cause Recommended action
Sample data files from the instrument can be
opened but not analyzed in secondary analysis
software
•
Sequencing Analysis Software
•
SeqScape
™
Software
•
Variant Reporter
™
Software
•
Minor Variant Finder Software
•
MicrobeBridge Software
•
GeneMapper
™
Software
An updated version of the
secondary analysis software
is required.
Download and run the latest
version of secondary analysis
software. See “Secondary
analysis software” on
page 50.
Note: In the secondary
analysis software, the
SeqStudio
™
Flex Series
Genetic Analyzer instrument
is listed as 3600.
Files cannot be imported into Thermo Fisher
™
Connect applications or cannot be analyzed in
Thermo Fisher
™
Connect sequencing apps
The sample name or file
name used a special
character.
Rename the sample name or
file name.
Note: Special characters (/,
&, @, %) will impact file
import into Thermo Fisher
™
Connect applications.
Spatial calibration troubleshooting
Observation Possible cause Recommended action
Spatial and spectral calibration
reports list original date of
capillary array installation (not
the date of re-installation)
— No action. Normal occurrence.
Persistently bad spatial
calibration results
The capillary array is damaged. Run the Change capillary array maintenance
wizard to uninstall, then re-install the
capillary array. (In the home screen, tap
Actions4Maintenance4Maintenance
wizards4Change capillary array.)
Contact Technical Support.
Unusual peaks or a flat line for
the spatial calibration
The capillary array window is
not properly positioned in the
detection cell (see Figure 3).
Open the detection block door and manually
reposition the detection cell within the holder.
Ensure that the fastener on the detection block
door is firmly tightened. Repeat the spatial
calibration.
Run the Change capillary array maintenance
wizard to uninstall, then re-install the
capillary array. (In the home screen, tap
Actions4Maintenance4Maintenance
wizards4Change capillary array.)
If the calibration fails again, run the
Fill array with polymer maintenance
wizard. (In the home screen, tap
Actions4Maintenance4Maintenance
wizards4Fill array with polymer.)
Appendix A Troubleshooting
Spatial calibration troubleshooting
A
522
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide