Table 18 Fragment analysis run modules (continued)
Run
module
type
Run module
name
Configuration 23 hours throughput
[1]
Performance
Cap.
length
(cm)
Pol. type
Run
time
(min)
(8-
cap.)
(24-
cap.)
Range
[2]
Sizing precision
[3]
50–
400bp
401–
600bp
601–
1,200
bp
Long
fragment
analysis
LongFragAnalysi
s50_POP7
LongFragAnalysi
s50_POP7xl
50 POP-7
™
≤125 ≥88 ≥264 ≥40 to
≤700
<0.15 <0.30 <0.45
SNaPshot
™
SNaPshot50_PO
P7
SNaPshot50_PO
P7xl
50 POP-7
™
≤30 ≥368 ≥1,104 ≥40 to
≤120
<0.50 NA NA
[5]
[1]
T
hroughput (samples/day): The total number of samples run in 23 hours (0.5 hour is required for user interaction and 0.5 hour is required for
warm-up time).
[2]
Resolution range: The range of bases over which the resolution (peak spacing interval divided by the peak width at half-maximum in a
GeneScan
™
600 LIZ
™
Size Standard or GeneScan
™
1200 LIZ
™
Size Standard sample sized with a third order fit) is ≥1. The table shows the
resolution range in ≥90% of samples.
[3]
Sizing precision: Standard deviation of sizes for one allele in the DS-33 install standard sized with the GS600 LIZ size standard across multiple
capillaries in the same run. For one injection to pass, 100% of the alleles in that injection must meet the intra-run sizing precision specifications.
The table shows the sizing precision of 100% of alleles in ≥90% of samples.
[4]
Throughput is an estimate extrapolated from average run time.
[5]
Not applicable because of the size of the fragments collected in the run.
Dye sets
Table 19 Sequence analysis dye sets
Dye set Application
E (v1.1 BigDye
™
Terminator) Rapid DNA sequencing
Z (v3.1 BigDye
™
Terminator) DNA sequencing
Table 20 Fragment analysis dye sets
Dye set Application
E5 SNaPshot
™
kit
G5 DNA sizing for 5-dye chemistry
J6 or J6-T DNA sizing for 6-dye chemistry
F DNA sizing for 4-dye chemistry
D DNA sizing for 4-dye chemistry
Appendix B Run modules and dye sets
Dye sets
B
530
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
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