QC errors—Spectral calibration (manual)
QC error
Description Action
Insucient number of dye spectra
detected.
An incorrect calibration standard or
setting was used for calibration.
•
Ensure that the matrix standard
or sequencing standard that you
use matches the dye set you
select for calibration.
•
Sequencing calibration—
–
Ensure that you select the
dye set from the Matrix tab
if you are calibrating with a
matrix standard.
–
Ensure that you select
the dye set from the
Sequencing tab if you
are calibrating with a
sequencing standard.
The calibration standard has
degr
aded or was incorrectly
prepared.
Figure 94 shows an example where
a peak is missing, possibly due to
degraded matrix standard.
Ensure that all of the following
conditions are met:
•
The calibration standard is not
expired.
•
The calibration standard is
prepared and stored as
specified in the product
information sheet for the
calibration standard.
•
The calibration plate was
prepared within 24 hours of the
calibration run.
•
Ensure that you used fresh
Hi‑Di
™
Formamide. See “Hi‑Di
™
Formamide storage and usage”
on page 28. Older formamide
can break down to generate
formic acid, which can degrade
the calibration standard.
•
Ensure that plate septa are not
being reused.
Appendix A Troubleshooting
QC error and alert troubleshooting
A
492
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
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