Thermo Scientific appliedbiosystems SeqStudio Flex Series - Page 491

(continued)
QC error Description Action
Sizing quality value is zero. Various causes. Check the data quality of
the size standard peaks, such
as the peak height and the
peak shape. See “Check
size standard quality (fragment
analysis)” on page 476.
The number of size standard peaks detected
is less than the number of peaks defined in
the size standard.
The size standard definition that
is specified in the plate file
includes peaks that are not
present in the sample.
Example: In a run that
uses GeneScan
™
600 LIZ
™
Size Standard, you used
the GS600LIZ(60-600) size
standard definition, but the
run module collects data
only up to 520 bp.
The GS600_LIZ_(60-460) size
standard definition could be
used instead.
•
Check the r
aw data to
ensure that size standard
peaks are present at
the expected peak height
(“Check size standard
quality (fragment analysis)”
on page 476).
•
Use or create a size
standard definition with
the appropriate number of
peaks and peak sizes.
•
Create a run module with a
longer run time.
•
If the run module has
previously detected the
appropriate size standard
peaks, ensure that the run
module was not edited to
reduce the run time.
The analysis range is too small. Correct the
analysis r
ange in analysis settings and re-
analyze in secondary analysis software or re-
inject sample.
Various causes.
•
Analyze the data in
a secondary analysis
software with a corrected
analysis range.
•
Re-inject the sample.
The sample does not contain the dye
specified in the size standard.
The incorrect size standard
definition is specified in the
plate file.
Use a size standard that
specifies all the dyes that are
present in a sample.
Appendix A Troubleshooting
QC error and alert troubleshooting
A
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
491