Thermo Scientific appliedbiosystems SeqStudio Flex Series - Avoiding Damage to the Pump Assembly; Remove Bubbles from the Polymer Pump (Wizard)

Avoiding damage to the pump assembly
The polymer delivery pump can be irreversibly damaged if any of the following conditions occur:
•
Polymer dries in the polymer channels of the pump assembly, which can cause blockage.
•
The pump assembly is exposed to organic solvent, which can cause cracking and clouding of the
acrylic pump material and valves.
•
The pump assembly is exposed to temperatures >40°C, which can damage the pump components.
•
There is arcing in the pump assembly, which can damage the acrylic pump material.
•
The water trap does not contain water. Polymer can escape past the pump seal and crystallize
inside the pump. Flush the water trap monthly. See “Flush the water trap (pump trap)” on page 379.
Remove bubbles from the polymer pump (wizard)
Before each run, check the polymer pump fluidic pathway for bubbles. If necessary, run the Remove
bubbles maintenance wizard.
Note: A polymer pouch includes a small reserve volume that is used for the Remove Bubbles
maintenance wizard, which consumes ~350 μL of polymer. The reserve volume is sucient to run the
wizard ~4 times (including the remove bubbles step during other maintenance wizards). If you manually
run the Remove Bubbles maintenance wizard >4 times, the volume of polymer that is available for
samples may be depleted.
1.
In the home screen, tap Actions4Maintenance4Maintenance wizards4Remove bubbles.
2.
Follow the wizard instructions to perform the procedure. See “Remove Bubbles maintenance
wizard” on page 409.
When instructed to examine the fluidic pathway, ensure that all bubbles are removed from the
following locations:
•
Pump channels (Figure 23)
•
Anode channels (Figure 24)
Chapter 13 Maintain the Instrument
Maintain the pump (wizards)
13
SeqStudio
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Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
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