Thermo Scientific appliedbiosystems SeqStudio Flex Series - APPENDIX B Run Modules and Dye Sets; Run Modules, Read Lengths, Size Ranges, and Run Times

Run modules and dye sets
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Run modules, read lengths, size ranges, and run times ................................... 526
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Dye sets ........................................................................... 530
Run modules, read lengths, size ranges, and run times
IMPORTANT! Use BDX run modules only if you prepare samples with the BigDye XTerminator
™
Purification Kit. Use non-BDX run modules for samples purified with other methods.
Table 17 Sequencing analysis run modules
Run module
type
Run module name
Configuration 23 hours throughput
[1]
Perform
- ance
Cap.
length
(cm)
Polymer
type
Run
time
(min)
(8‑cap.) (24-cap.)
Contig.
Read
L
ength
(CRL)
[2]
Short read
sequencing
ShortReadSeq50_POP7
ShortReadSeq50_POP7xl
50 POP-7
™
≤30 ≥368 ≥1,104 ≥300
Short read
sequencing
BigDye
XTerminator
™
BDxShortReadSeq50_POP7
BDxShortReadSeq50_POP7xl
50 POP-7
™
≤30 ≥368 ≥1,104 ≥300
Rapid
sequencing
RapidSeq50_POP6
RapidSeq50_POP6xl
50 POP-6
™
≤65 ≥168 ≥504 ≥450
RapidSeq50_POP7
RapidSeq50_POP7xl
50 POP-7
™
≤40 ≥280 ≥840 ≥500
Rapid
sequencing
RapidSeq36_POP4
RapidSeq36_POP4xl
36 POP-4
™
≤45 ≥240 ≥720 ≥400
RapidSeq36_POP6
RapidSeq36_POP6xl
36 POP-6
™
≤70 ≥168 ≥504 ≥600
RapidSeq36_POP7
RapidSeq36_POP7xl
36 POP-7
™
≤30 ≥368 ≥1,104 ≥600
B
526
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide