Figure 16 Spectral calibration (left top), install run (left bottom), and regular run (right) dye set and
chemistr
y selections when using the BigDye
™
Direct Cycle Sequencing Kit
Estimated spectral calibration run times
Note: The run times list
ed below do not include the time for the oven to preheat.
Standard
Polymer type and capillary array configur
ation
Approximate run time
POP-4
™
36 cm
POP-4
™
50
cm
36 cm 50 cm
POP-7
™
36
cm
POP-7
™
50
cm
Sequencing standard 36 minutes Not
supported
65 minutes 95 minutes 62 minutes 52 minutes
Sequencing or fragment analysis
matrix standard
28 minutes 44 minutes 63 minutes 27 minutes 33 minutes
Note: A sequencing standar
d can be used for a sequencing install run. A spectral calibration can also
be obtained from the sequencing install run. The sequencing standard contains DNA that has been
sequenced with a BigDye
™
chemistry. The spectral calibration is obtained from the sequencing install
run by evaluating the dyes associated with the 4 nucleotides in the sequencing standard.
A sequencing matrix standard contains 4 nucleotide/dye combinations, with one labeled peak per dye
label, and is used for spectral calibration.
Chapter 12 Run calibrations and install checks
Run a spectral calibration
12
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
313
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