Thermo Scientific appliedbiosystems SeqStudio Flex Series - Sample and Data Troubleshooting

Observation Possible cause Recommended action
Unable to connect to SAE
server message
The SAE server connection
settings are incorrect.
1.
Check the SAE server IP address (see
“Determine the IP address for a computer
on a network” on page 443).
2.
In the instrument Sign In screen, sign in
with a local administrator profile.
3.
Set the correct IP address (in the home
screen, tap Actions4Settings4 SAE
settings4Connection settings).
There is a problem with
the computer on which the
SAE Administrator Console is
installed or a problem with the
network.
Troubleshoot computer or network problems.
The computer on which the
SAE Administrator Console is
installed has a dynamic IP
address that is disconnecting
the server when the computer
is restarted.
Set a static IP address on the computer.
Firewall settings are not
correctly set.
Contact Technical Support.
The Open file from non-SAE
system Setting from SAE
server setting in the SAE
Administrator Console does
not change the software
behavior
The Open file from non-
SAE system Setting from
SAE server function is not
supported in this software.
No action.
Sample and data troubleshooting
Observation Possible cause Recommended action
"Calibration from sample" is
reported for spectral calibration
status
Auto-spectral calibration is run
on all samples and generates
the status.
See “Auto-spectral calibration
during an injection” on
page 150.
No action. Normal occurrence.
"Calibration from manual" is
reported for spectral calibration
status, but no pull-up peaks
are observed
Auto-spectral calibration was
not used. For more information,
see “Auto-spectral calibration
during an injection” on
page 150.
No action. Normal occurrence.
Appendix A Troubleshooting
Sample and data troubleshooting
A
512
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide