Thermo Scientific appliedbiosystems SeqStudio Flex Series - Prepare for Custom Dye Set Spectral Calibration; Prepare for Spectral Calibration

Prepare for custom dye set spectral calibration
Before you begin, create a custom dye set in the dye set library. See “Create a custom dye set” on
page 286.
Prepare for spectral calibration
Prepare the instrument
1.
Display the consumables dashboard by tapping (Dashboard) at the top right of the home
screen (if the consumables dashboard is not displayed, tap or ).
2.
Check consumables status (see “Check consumables status” on page 66). Ensure that:
•
Consumables are not expired
•
Adequate injections remain for consumables
IMPORTANT! F
or install runs, use polymer that has been on the instrument ≤3 days. Slight peak
shifts can occur with polymer that has been on the instrument >3 days.
3.
Ensur
e that buer levels are at the fill lines (see “Check buer fill levels” on page 70).
4.
Check the oven and detection cell temperature to ensure they are at the correct temperature (see
“Preheat the instrument oven and the detection cell” on page 68).
5.
Check the pump assembly for bubbles and run the Remove Bubble wizard if needed (see
“Remove bubbles from the polymer pump (wizard)” on page 377).
Prepare the spectral calibration standard
For the instructions to prepare the standard, see the product information sheet. To find the product
information sheet, go to www.thermofisher.com, search for the product, then scroll to the bottom of
the product page.
Prepare the matrix standard appropriate for your application as described in the product insert.
Note: If you use the BigDye
™
Direct Cycle Sequencing Kit, perform the spectral calibration with
BigDye
™
v3.1 matrix standards. For more information, see “Using BigDye
™
Direct Cycle Sequencing Kit
chemistry” on page 312.
Name Cat. No.
DS-02 Matrix Standard Kit (Dye Set E5) 4323014
DS-30 Matrix Standard Kit (Dye Set D) 4345827
DS-31 Matrix Standard Kit (Dye Set D with VIC
™
dye) 4345829
DS-32 Matrix Standard Kit (Dye Set F) 4345831
DS-33 Matrix Standard Kit (Dye Set G5) 4345833
DS-36 Matrix Standard Kit (Dye Set J6) 4425042
DS-37 Matrix Standard Kit (Dye set J6‑T
, 6‑dye) A31234
Chapter 12 Run calibrations and install checks
Run a spectral calibration
12
314
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide