Thermo Scientific appliedbiosystems SeqStudio Flex Series - Page 294

Setting Description
Clear range methods
•
Use quality values—Sets a window with a specified number of allowed
low-quality bases by r
emoving bases until there are <X number of
bases per Z number of bases with QV <Y.
•
Use base positions—Specifies the first and last basecalls in the range
to consider, or trims the specified number of basecalls from the 3′ end.
•
Use identification of N calls—Sets a window with a specified number
of allowed ambiguous basecalls (Ns) by removing bases until there are
<X number of Ns per Y number of bases.
Chapter 11 Manage library resources on the instrument
Analysis settings library
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SeqStudio
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Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide