1
Reference sequence of the sequencing install standard
2
Basecalls from the run. Basecalls that do not match the known bases in the reference sequence are marked in
r
ed.
3
QV20 CRL is r
eferred to as CRL on the Capillaries tab.
4
Alignment r
ange. The starting base is determined by the software. In the example above, the alignment range
starts at 27 because of the contiguous bases before 27 that do not match the reference sequence.
5
Accuracy is r
eferred to as %RL basecall on the Capillaries tab.
Chapter 12 Run calibr
ations and install checks
Perform an install run
12
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
339
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