Thermo Scientific appliedbiosystems SeqStudio Flex Series - Page 4

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CHAPTER 2 Get started .......................................................... 53
Sign in ........................................................................ 53
Profile and sign in options ....................................................... 55
Create a local profile (one time only) .......................................... 55
Sign in ................................................................... 57
Switch user or sign out .................................................... 59
Sign in (if another user is signed in) .......................................... 60
Workflow: Create a plate, start and monitor a run, then view results .................. 62
Prepare the instrument .......................................................... 64
Review upcoming maintenance .............................................. 64
Check consumables status ................................................ 66
Check instrument status ................................................... 67
Preheat the instrument oven and the detection cell ............................. 68
Check for leaks and spills ................................................... 68
Check buer fill levels ...................................................... 70
Replenish consumables .................................................... 71
Ensure proper installation of CBC septa ...................................... 71
Control the LED light, speaker volume, and screen brightness ................... 72
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CHAPTER 3 Prepare the samples ................................................ 73
Sample preparation guidelines ................................................... 73
Plate and strip-tube layout ...................................................... 75
Prepare and assemble sample plates ............................................. 76
Prepare sample plates ...................................................... 76
Prepare the plate assembly ................................................. 77
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CHAPTER 4 Create a plate file on the instrument ............................. 83
Create, copy, or import a plate file ............................................... 83
Options for creating plate files ............................................... 83
Create a plate file .......................................................... 84
Copy a plate file ........................................................... 85
Import a plate file .......................................................... 86
Enter plate properties ........................................................... 88
Enter injection properties ........................................................ 89
Specify replicate injections ...................................................... 92
Enter sample names, sample types, and custom fields .............................. 93
Contents
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SeqStudio
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Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide