Thermo Scientific appliedbiosystems SeqStudio Flex Series - Page 516

Observation Possible cause Recommended action
Pull-up or pull-down peaks are
present in the data
(continued)
Auto-spectral calibration was
not used because the sample
possibly contains overlapping
peaks that are labelled with
dierent dyes. For more
information, see “Auto-spectral
calibration during an injection”
on page 150.
Perform a manual spectral calibration, then
repeat the run. See “Run a spectral
calibration” on page 310.
Noise is present in the baseline
Fluorescent contamination has
built up in the CBC.
Replace the CBC. Use new CBC septa when
assembling the new CBC.
The plate septum has been
used more than one time and
has introduced contamination.
Repeat the run with a new plate septum.
Contamination from a source
other than the CBC is present.
Wash the pump. See “Wash the pump
chamber and channels (wizard)” on page 379.
Install fresh consumables.
Sample carryover from a
previous injection
Sample carryover can occur
in trace amounts when
previous injections are o-
scale. Very strong peaks, for
example, primer peaks in a
fragment analysis sample, are
typically visible in subsequent
injections.
Replace the CBC septa before the next
injection to minimize the carryover eect.
If sample carryover is from samples with signal
>25,000–30,000 RFU, consider diluting the
sample.
Poor resolution in some
capillaries
Poor-quality samples were
used.
See “Sample preparation guidelines” on
page 73.
Use a control sample to determine if the poor
resolution is due to the samples or another
factor.
A capillary was damaged or is
blocked.
Run the Fill array with polymer
maintenance wizard to clear the
capillary. (In the home screen, tap
Actions4Maintenance4Maintenance
wizards4array with polymer.)
If the poor resolution is due to the
capillary array and not the sample, run
the Change capillary array maintenance
wizard. (In the home screen, tap
Actions4Maint
enance4Maintenance
wizards4Change capillary array.)
Poor resolution in all the
capillaries
The polymer has been used for
mor
e than the stated number
of injections, it is past the
labeled expiration date, or
has degraded polymer from
incorrect storage.
Install fresh polymer with the
Replenish polymer maintenance wizard.
(In the home screen, tap
Actions4Maintenance4Maintenance4
wizards4Replenish polymer.)
Appendix A Troubleshooting
Sample and data troubleshooting
A
516
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide