Thermo Scientific appliedbiosystems SeqStudio Flex Series - Page 10

Example spatial profiles ................................................... 308
Export spatial calibration results or report .................................... 309
Run a spectral calibration ...................................................... 310
Overview of spectral calibration ............................................ 310
When to perform a spectral calibration ...................................... 311
System dye sets versus custom dye sets .................................... 312
Using BigDye
™
Direct Cycle Sequencing Kit chemistry ........................ 312
Estimated spectral calibration run times ..................................... 313
Prepare for custom dye set spectral calibration .............................. 314
Prepare for spectral calibration ............................................. 314
Perform a spectral calibration .............................................. 316
View spectral calibration results ............................................ 317
Example spectral calibration data ........................................... 324
Export spectral calibration logs or report .................................... 325
View and export the spectral calibration history .............................. 326
Perform an install run .......................................................... 328
Overview of install run ..................................................... 329
When to perform an install run ............................................. 329
Estimated install run times ................................................. 330
Prepare for the install run .................................................. 330
Perform an install run ..................................................... 333
View sequencing install run results .......................................... 334
View fragment analysis install run results .................................... 347
View, export, or delete an install run history ................................. 352
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CHAPTER 13 Maintain the Instrument ......................................... 354
Maintenance schedule ......................................................... 354
Review upcoming maintenance ............................................ 355
Daily instrument maintenance tasks ......................................... 356
Monthly instrument maintenance tasks ...................................... 357
Annual planned maintenance tasks ......................................... 358
As-needed instrument maintenance tasks ................................... 358
Maintenance wizard overview .................................................. 359
Clean the instrument .......................................................... 361
Export consumables log files ................................................... 363
Install buers (wizard) ......................................................... 363
Install the anode buer container (ABC) ..................................... 364
Install the cathode buer container (CBC) ................................... 367
Replenish, change, flush, and store polymer (wizards) ............................. 370
Precautions for use ....................................................... 370
Replenish polymer or change polymer type (wizard) ........................... 370
Store partially used polymer ............................................... 371
Contents
10
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide