Description Setting
Size calling method
•
Local Southern (default)—Determines the fragment sizes using the reciprocal
relationship between fragment length and electrophoretic mobility.
•
Global Southern—Compensates for standard fragments with anomalous
electrophoretic mobility (similar to least squares methods).
•
Second Order Least Squares—Uses regression analysis to build a best-fit size
calling curve.
•
Third Order Least Squares—Uses regression analysis to build a best-fit size
calling curve.
•
Cubic Spline—Forces the sizing curve through all the known points of the
selected size standard.
Chapter 11 Manage library resources on the instrument
Analysis settings library
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SeqStudio
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Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
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