Thermo Scientific appliedbiosystems SeqStudio Flex Series - Page 321

Spectral QV and Condition number limits
Dye set Minimum spectral QV
Matrix condition number upper
limit
D (DS-30) 0.95 8.5
D (DS-31) 0.95 8.5
E_BigDye Terminator v1.1
[1]
0.95 5.5
E5 (DS-02) 0.95 6.0
F (DS-32) 0.95 8.5
G5 (DS-33) 0.95 13.5
J6 (DS-36) 0.95 8.0
J6-T (DS-37) 0.95 8.0
Z_BigDye Terminator v3.1
[1]
0.95 5.5
Z_BigDye Direct
[2]
0.95 5.5
[1]
T
he E_BigDye Terminator v1.1 limits are also used for the optional spectral calibration during sequencing install run. Borrowing is
automatically enabled for the optional calibration.
[2]
For information on the Z_BigDye Direct dye set, see “Using BigDye
™
Direct Cycle Sequencing Kit chemistry” on page 312.
Spectral calibration sharing between capillaries
If spectral borrowing is enabled, a spectral calibration can share capillary information:
•
Between injections (only when Auto-retry is enabled)—If a capillary in an injection does not meet
the Spectral QV (quality value) and Condition number limits, the software automatically uses the
spectral calibration from that capillary in a dierent injection.
•
Within an injection—If a capillary in an injection does not meet the Spectral QV (quality value) and
Condition number limits and the Allow borrowing option is selected, the software can also use
the spectral calibration from a capillary to the left, right, above, or below that capillary, if the values
are more optimal than those for that capillary in a dierent injection.
Chapter 12 Run calibr
ations and install checks
Run a spectral calibration
12
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
321