Spectral calibration with Allow borrowing disabled
Table 9 Overview of spectral calibration options with Allow borrowing disabled
Option Action
Allow borrowing
disabled
Auto Retry disabled
•
All capillaries must pass (must meet the Spectral QV and Condition
number limits set internally in the software).
•
One run is performed (see Injection 1 below).
•
If the limits are not met, the calibration fails.
This option is not illustrated in the table below.
Allow borrowing
disabled
Aut
o Retry enabled
Same as above, except up to 3 runs are performed (see Injections 1 through 3
below).
Table 10 Details of spectral calibration injections with Allow borrowing disabled and Auto Try
enabled
Injection Process
Injection 1
•
The softwar
e evaluates the Spectral QV and Condition number of all capillaries.
•
If all capillaries pass, the calibration is complete, and injections 2 and 3 are not
performed.
•
If any capillaries fail and Auto Retry is disabled, the calibration fails.
If any capillaries fail and Auto Retry is enabled, injection 2 is performed.
Injection 2 (listed as
re-injection in
Run queue)
•
The softwar
e evaluates the Spectral QV and Condition number for each capillary
across injections 1 and 2 and uses the information from the capillary with the
highest Spectral QV.
•
If all capillaries now pass, the calibration is complete and injection 3 is not
performed.
•
If the same capillary fails in both injection 1 and 2, injection 3 is performed.
Injection 3 (listed as
re-injection in
Run queue)
•
The softwar
e evaluates the Spectral QV and Condition number for each capillary
across injections 1, 2, and 3 and uses the information from the capillary with the
highest Spectral QV.
•
If all capillaries now pass, the calibration passes.
•
If the same capillary fails in injection 1, 2, or 3 and the calibration fails.
Chapter 12 Run calibrations and install checks
Run a spectral calibration
12
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
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